Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

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Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain ...

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The suprachiasmatic nucleus (SCN), site of the dominant mammalian circadian clock, contains a variety of different neurons that tend to form groups within the nucleus. Somatostatin was first discovered in hypothalamic extracts and identified as a hormone that inhibited secretion of growth hormone. Subsequently, somatostatin was found to be secreted by a broad range of tissues, including pancrea...

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Simple Method For Stationary Organotypic Slice Culture of Hypothalamic Suprachiasmatic Nucleus

Early postnatal brain tissue can be cultured in viable and healthy condition for several weeks with development and preservation of the basic cellular and connective organization as socalled organotypic brain slice cultures. The present study describes a simple method for stationary long-term organotypic culture for the hypothalamic suprachiasmatic nucleus (SCN). Hypothalamic brain slices from ...

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Orexin depolarizes rat hypothalamic paraventricular nucleus neurons.

Orexins, also called hypocretins, are newly discovered hypothalamic peptides that are thought to be involved in various physiological functions. In spite of the fact that orexin receptors, especially orexin receptor 2, are abundant in the hypothalamic paraventricular nucleus (PVN), the effects of orexins on PVN neurons remain unknown. Using a whole cell patch-clamp recording technique, we inves...

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ژورنال

عنوان ژورنال: Journal of Veterinary Science

سال: 2007

ISSN: 1229-845X

DOI: 10.4142/jvs.2007.8.1.15